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Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:08:28.096
Product last modified at: 2025-03-28T20:00:13.903Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

p18 INK4C (DCS118) Mouse mAb #2896

Filter:
  • WB
  • IP
Western Blotting Image 1: p18 INK4C (DCS118) Mouse mAb
Western blot analysis of extracts from HeLa, 293 and Ramos cells, using p18 INK4C (DCS118) Mouse mAb.

To Purchase # 2896

Cat. # Size Qty. Price
2896T 20 µl
$143
2896S 200 µl
$336

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 18
Source/Isotype Mouse IgG2a
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

The p18 INK4C (DCS118) Mouse mAb detects endogenous levels of p18 INK4C protein. The antibody does not cross-react with other proteins of the INK4 family.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with purified recombinant human p18 INK4C protein.

Background

Cyclin-dependent kinases (CDKs) are activated in part by forming complexes with cyclins. For example, CDK4 and CDK6 associate with the D-type cyclins and phosphorylate the retinoblastoma protein. This phosphorylation is a necessary event for cells to enter S-phase (1). The inhibitors of CDK4 (INK4) family include p15 INK4B, p16 INK4A, p18 INK4C and p19 INK4D. p18 has been shown to function as a haploinsufficient tumor suppressor in vivo (2). All INK4 proteins are composed of 32 amino acid ankyrin motifs and selectively inhibit CDK4/6 activity. Mutational analyses of p18 implicate the third and the amino-terminal portion of the fourth ankyrin repeat in mediating binding to CDK4/6 (3). The interaction of INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression (4,5).

Pathways

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For Research Use Only. Not For Use In Diagnostic Procedures.
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