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Product last modified at: 2025-01-01T09:01:29.297Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

p22phox (E9N8W) Rabbit mAb #88048

Filter:
  • WB
  • IP
Western Blotting Image 1: p22phox (E9N8W) Rabbit mAb
Western blot analysis of extracts from various cell lines using p22phox (E9N8W) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 88048

Cat. # Size Qty. Price Ships
88048T 20 µl
$153
88048S 100 µl
$339

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 22
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

p22phox (E9N8W) Rabbit mAb recognizes endogenous levels of total p22phox protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro187 of human p22phox protein.

Background

The p22phox protein is a critical component of the membrane-bound NADPH Oxidase of phagocytes that generate superoxide anion and alter the redox states in cells. p22phox associates with the catalytic subunit gp91phox (NOX2), NOX1, NOX3, or NOX4 to form the active NADPH Oxidase. The enzymatic complex includes cytosolic subunits p40phox, p47phox, p67phox, and Rac2 that are recruited to the membrane-bound subunits, subsequently generating superoxide anion as the final product, serving as a microbial killing system (1,2). Multiple phosphorylation sites in each subunit of these oxidases potentiate its activation, including the phosphorylation of p22phox at Thr147 epitope facing the cytoplasmic space (3-5). NADPH Oxidase is associated with several diseases, including hyperglycemia, cancer, inflammation and vascular cases, as well as neurodegenerative diseases (6), including its activation by SOD1 mutants in Amyotrophic Lateral Sclerosis (7), and in Alzheimer's disease where NADPH Oxidase activation exacerbates the effects of fibrillary tangles and β-amyloid plaques, causing neuronal death (8,9).
For Research Use Only. Not For Use In Diagnostic Procedures.
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