Render Target: STATIC
Render Timestamp: 2024-12-13T11:30:22.531Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:57:01.079
Product last modified at: 2024-11-15T07:00:09.696Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

p38α MAPK (L53F8) Mouse mAb #9228

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  • WB

    Supporting Data

    REACTIVITY H M R Mk Pg Sc
    SENSITIVITY Endogenous
    MW (kDa) 40
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Pg-Pig 
    • Sc-S. cerevisiae 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    p38α MAP Kinase (L53F8) Mouse mAb detects endogenous levels of total p38α MAPK. This antibody does not cross-react with either JNK/SAPK or p42/44 MAPK or other isoforms of p38.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, Pig, S. cerevisiae

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant p38 MAPK protein.

    Background

    p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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