p58IPK (C56E7) Rabbit mAb #2940
- WB
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 58 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
p58IPK (C56E7) Rabbit mAb detects endogenous levels of total p58IPK protein.
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp127 of human p58IPK.
Background
p58IPK is an inhibitor of interferon-induced and double-stranded RNA-activated protein kinase (PKR). It physically interacts with PKR and inhibits its activation and activity (1). Influenza virus activates p58IPK and thus blocks the activity of PKR to repress translation in the infected cells. In the uninfected cells, p58IPK forms a complex with its own inhibitor, HSP40, and is kept in an inactive state (2). ER stress induces the expression of p58IPK mediated by ATF6 (3,4). The induced p58IPK negatively regulates Perk activity, inhibits eIF2α phosphorylation and suppresses the activation of expression of downstream ER-responsive genes ATF4 and GADD153 (4). More recently, p58IPK has been shown to associate with the lumen of the endoplasmic reticulum (ER) where it is believed to serve as a cochaperone for BiP (5).
Limited Uses
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