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Render Timestamp: 2024-11-26T11:18:49.856Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-09-30 01:54:11.024
Product last modified at: 2024-11-20T13:00:36.282Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. p73 (D3G10) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

p73 (D3G10) Rabbit mAb #14620

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    p73 (D3G10) Rabbit mAb recognizes endogenous levels of total p73 protein. Based on the amino acid sequence of the immunogenic peptide, the antibody is expected to recognize all p73 isoforms.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala80 of human p73 protein.

    Background

    The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator (1-3). Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix (4). DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 (5). Another report, however, indicates that p300 does not acetylate full-length p73 in vivo (6).

    Database Links

    UniProt ID: O15350


    Entrez-Gene Id: 7161


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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