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Render Timestamp: 2024-11-20T12:04:51.939Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:54:20.460
Product last modified at: 2024-11-14T14:00:43.221Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. p95/NBS1 (D6J5I) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

p95/NBS1 (D6J5I) Rabbit mAb #14956

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 95
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    p95/NBS1 (D6J5I) Rabbit mAb recognizes endogenous levels of total p95/NBS1 protein. This antibody also cross-reacts with an unidentified protein of 180 kDa in some cell lines.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala740 of human p95/NBS1 protein.

    Background

    Nijmegen breakage syndrome (NBS) is characterized by growth retardation, mental disability, immunodeficiency, defects in cell cycle checkpoints, an increased propensity for cancer, and sensitivity to ionizing radiation (1). Repair of radiation-induced DNA double-strand breaks is dependent on the multifunctional MRN complex containing Mre11, Rad50, and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2). p95/NBS1 is a protein with a forkhead-associated domain and a BRCT repeat that regulate interaction with MDC1 and are essential for proper G2/M DNA-damage checkpoint function (3). NBS1 is critical for homologous recombination following DNA double-strand breaks. This activity requires CDK-dependent association with CtIP and subsequent phosphorylation by ATM (4). ATM interacts with and phosphorylates p95/NBS1 at Ser278 and Ser343 after exposure to ionizing radiation (5,6).

    Database Links

    UniProt ID: O60934


    Entrez-Gene Id: 4683


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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