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Render Timestamp:
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Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:11:37.504
Product last modified at: 2025-04-02T12:45:10.663Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PAF15 (D8E2Y) XP® Rabbit mAb #81533

Filter:
  • WB
  • IHC
Western Blotting Image 1: PAF15 (D8E2Y) XP® Rabbit mAb
Western blot analysis of extracts from HT-29 and A172 cells, untreated or treated with aphidicolin (10 μg/mL, 24 hr) to enrich cells in S phase, using PAF15 (D8E2Y) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 81533

Cat. # Size Qty. Price
81533T 20 µl
$199
81533S 100 µl
$463

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 15
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:150

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

PAF15 (D8E2Y) XP® Rabbit mAb recognizes endogenous levels of total PAF15 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PAF15 protein.

Background

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). PCNA associated factor (PAF15) interacts with PCNA to recruit DNA replicative polymerase. In response to DNA damage, PCNA recruits a low fidelity DNA polymerase to allow bypass of lesions that would otherwise stall DNA replication. This form of DNA damage tolerance is called translesion synthesis (TLS), and is regulated in part by PAF15 (2,3). Ubiquitination of PAF15 at lysines 15 and 24 regulates its interactions with PCNA and DNA polymerase (3). PAF15 is overexpressed in human cancers, and research studies show a correlation between PAF15 expression and poor prognosis (3,4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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