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Pan-Actin (C4) Mouse mAb

Pan-Actin (C4) Mouse mAb #41185

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	45
Source/Isotype	Mouse IgG1

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:5000

Storage

Supplied as unpurified ascites fluid containing 10mM sodium azide. Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance. This product is stable for 36 months when stored at -20C.

Protocol

Specificity / Sensitivity

Pan-Actin (C4) Mouse mAb recognizes endogenous levels of total actin protein. This antibody reacts with all actin isoforms.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

This monoclonal antibody is unpurified ascites fluid produced by immunizing animals with chicken gizzard actin.

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids (2). For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (3). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (3). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (4). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (5-7). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (7).

Database Links

UniProt ID: P60709 , P68133 , P63261 , P68032 , P62736 , P63267

Entrez-Gene Id: 60 , 58 , 71 , 70 , 59 , 72

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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