Render Target: STATIC
Render Timestamp: 2024-12-20T12:21:23.000Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:53:44.714
Product last modified at: 2024-12-17T18:47:59.946Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Pan-TEAD (D3F7L) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Pan-TEAD (D3F7L) Rabbit mAb #13295

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 50, 53, 55, 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Pan-TEAD (D3F7L) Rabbit mAb recognizes endogenous levels of total TEAD proteins. This antibody has been shown to recognize TEAD 1, 2, 3 and 4 in transfected cell extracts.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys65 of human TEAD1 protein.

    Background

    The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of Yes-associated protein (YAP), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). When the Hippo pathway is turned on, YAP is phosphorylated by LATS1/2 kinase and sequestered in the cytoplasm by 14-3-3 protein binding, rendering YAP inactive. When the Hippo pathway is off, non-phosphorylated YAP translocates to the nucleus where it associates with various transcription factors including members of the transcriptional enhancer factor (TEF) family, also known as the TEA domain (TEAD) family (TEAD1-4) (5,6). Although widely expressed in tissues, the TEAD family proteins have specific tissue and developmental distributions. YAP/TEAD complexes regulate the expression of genes involved in cell proliferation and apoptosis (5).

    Database Links

    UniProt ID: Q15562 , P28347 , Q15561 , Q99594


    Entrez-Gene Id: 8463 , 7003 , 7004 , 7005


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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