Pannexin-1 (D9M1C) Rabbit mAb #91137
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-55, 19 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Pannexin-1 (D9M1C) Rabbit mAb recognizes endogenous levels of total pannexin-1 protein. This antibody detects an amino-terminal pannexin-1 fragment produced by caspase cleavage.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human pannexin-1 protein.
Background
The pannexin family (pannexin-1, -2, and -3; PANX1-3) of gap junction proteins has homology to the invertebrate innexins and display distinct expression patterns (1). Pannexin-1 is widely expressed, with highest expression in the heart, brain, skeletal muscle, testis, and ovary (1,2). Pannexin-2 is predominately expressed in the brain (1,2) and pannexin-3 is found within the skin and connective tissues (1,3). Connexin family gap junction proteins form hemichannels that align adjacent cells, creating functional intercellular channels that are permeable to ions and small molecules. In contrast, pannexin proteins may not function as gap junction proteins since pannexins on adjacent cells may not align to form complete channels (3). These pannexin “hemichannels” may play a role in inflammation, apoptosis, and neuronal signaling by allowing permeability of ions, ATP, and potentially other small molecules into the extracellular space (4-6). Pannexin-1 can be activated by effector caspases (caspase-3 and -7), which leads to release of signal molecules that promote phagocytosis of apoptotic cells (7).
- Baranova, A. et al. (2004) Genomics 83, 706-16.
- Bruzzone, R. et al. (2003) Proc Natl Acad Sci U S A 100, 13644-9.
- Penuela, S. et al. (2007) J Cell Sci 120, 3772-83.
- Qu, Y. et al. (2011) J Immunol 186, 6553-61.
- Silverman, W.R. et al. (2009) J Biol Chem 284, 18143-51.
- MacVicar, B.A. and Thompson, R.J. (2010) Trends Neurosci 33, 93-102.
- Chekeni, F.B. et al. (2010) Nature 467, 863-7.
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