Render Target: STATIC
Render Timestamp: 2024-12-20T11:22:18.881Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:28:35.575
Product last modified at: 2024-06-27T13:37:03.314Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. PAPSS1 Antibody

PAPSS1 Antibody #96753

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 70
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PAPSS1 Antibody recognizes endogenous levels of total PAPSS1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro255 of human PAPSS1 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    PAPS Synthase 1 and 2 (PAPSS1, PAPSS2) are bifunctional enzymes with both ATP sulfurylase and APS kinase activity. They mediate two reactions to generate 3'-phosphoadenosine-5'-phosphosulfate (PAPS), a critical intermediate in the sulfate activation pathway. PAPS is the sulfate donor co-substrate for all sulfotransferase (SULT) enzymes and is required to catalyze the sulfate conjugation of many endogenous and exogenous compounds (1,2). For example, PAPSS1 and PAPSS2 are responsible for catalyzing the sulfation of steroid hormones, which increases their water solubility and reduces their biological activity, suggesting modulation of PAPS synthases as a possible avenue for treatment of hormone-dependent cancers (3). Inhibition of PAPSS1 was also shown to increase the sensitivity of some cancer cell lines to DNA-damage agents both in vitro and in vivo (4,5), suggesting further potential utility for PAPS synthase inhibitors in cancer therapy.

    Database Links

    UniProt ID: O43252


    Entrez-Gene Id: 9061


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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