Pax6 (D3A9V) XP® Rabbit mAb #60433
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunohistochemistry (Paraffin) | 1:250 - 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #58677.
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Pax6 has important functions in organ development. It is a key regulator of eye development (5), and mutations in Pax6 have been associated with some forms of aniridia, a congenital malformation of the eye (6). Pax6 is also involved in neuronal development, which plays an especially important role in corticogenesis (7). Within its role in the adult brain, it has recently been associated with aging, with gene occupancy studies showing increased association of Pax6 with genes associated with many aging processes in mice (8,9). Pax6 also plays another important function in development and maintenance of pancreatic β-cells (10). Driven by expression of Pdx1, both Pax6 and NGN3 expression is required for β-cell identity (11).
- Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
- Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
- Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
- Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.
- Shaham, O. et al. (2012) Prog Retin Eye Res 31, 351-76.
- Hingorani, M. et al. (2012) Eur J Hum Genet 20, 1011-7.
- Manuel, M.N. et al. (2015) Front Cell Neurosci 9, 70.
- Maurya, S.K. and Mishra, R. (2017) Ann Neurosci 24, 20-25.
- Maurya, S.K. and Mishra, R. (2017) J Chem Neuroanat 82, 60-64.
- Swisa, A. et al. (2017) Front Genet 8, 21.
- Mitchell, R.K. et al. (2017) J Biol Chem 292, 8892-8906.
Limited Uses
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