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Paxillin Antibody #2542

Filter:
  • WB
Western Blotting Image 1: Paxillin Antibody
Western blot analysis of extracts from various cell lines, using Paxillin Antibody.

To Purchase # 2542

Cat. # Size Qty. Price
2542S 100 µl
$306

Product Specifications

REACTIVITY H M R Mk B Pg
SENSITIVITY Endogenous
MW (kDa) 68
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • B-Bovine 
  • Pg-Pig 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Paxillin Antibody detects endogenous levels of total paxillin protein. This antibody does not cross-react with related proteins.

Species Reactivity:

Human, Mouse, Rat, Monkey, Bovine, Pig

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human paxillin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Paxillin is a multidomain protein that localizes primarily to focal adhesion sites in the extracellular matrix (1). Paxillin is one of the key components of integrin signaling, and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-paxillin (Tyr118) may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).

Pathways

Explore pathways related to this product.


For Research Use Only. Not for Use in Diagnostic Procedures.
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