PCAF (C14G9) Rabbit mAb #3378
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 93 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:25 |
| Chromatin IP-seq | 1:25 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
- Nagy, Z. and Tora, L. (2007) Oncogene 26, 5341-57.
- Yang, X.J. et al. (1996) Nature 382, 319-24.
- Schiltz, R.L. et al. (1999) J Biol Chem 274, 1189-92.
- Bannister, A.J. and Miska, E.A. (2000) Cell Mol Life Sci 57, 1184-92.
- Liu, L. et al. (1999) Mol Cell Biol 19, 1202-9.
- Martínez-Balbás, M.A. et al. (2000) EMBO J 19, 662-71.
- Sartorelli, V. et al. (1999) Mol Cell 4, 725-34.
- Imhof, A. et al. (1997) Curr Biol 7, 689-92.
- Munshi, N. et al. (1998) Mol Cell 2, 457-67.
- Herrera, J.E. et al. (1999) Mol Cell Biol 19, 3466-73.
Limited Uses
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