Render Target: STATIC
Render Timestamp: 2024-12-27T11:36:34.114Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-25 15:16:09.485
Product last modified at: 2024-12-13T21:30:09.453Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

PCM-1 (G2000) Antibody #5213

Filter:
  • WB
  • IP
  • IF
  • F

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 228
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:800
    Flow Cytometry (Fixed/Permeabilized) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PCM-1 (G2000) Antibody detects endogenous levels of PCM-1 protein. Species cross-reactivity for IF-IC is human only.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region near the carboxyl terminus of human PCM-1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

    Background

    The centrosome is composed of a pair of centrioles surrounded by electron-dense pericentriolar material and functions as the microtubule-organizing center responsible for microtubule nucleation and spindle organization during cell cycle progression (1). Pericentriolar material 1 (PCM-1) is a large, 228 kDa protein associated with the centrosome in a cell cycle-dependent manner (2). PCM-1 localizes to small cytoplasmic granules called centriolar satellites (3). PCM-1 is required for the assembly of several centrosomal proteins, including centrin, pericentrin, ninein, NEK2, and CEP250 (4-8). Chromosomal translocations involving genes encoding PCM-1 and the tyrosine kinases Ret and Jak2 are associated with some cancers, including papillary thyroid carcinoma and myeloid leukemia (9-11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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