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Product Name

86163 PD-1 (Intracellular Domain) (D4W2J) XP^® Rabbit mAb

Filter:

F

Inquiry Info. # 15121

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	40-50
Source/Isotype	Rabbit IgG

Application Key:

F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Flow Cytometry (Fixed/Permeabilized)	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

PD-1 (D3W4U) Rabbit mAb recognizes endogenous levels of total PD-1 protein. This antibody binds the intracellular domain of human PD-1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala249 of human PD-1 protein.

Background

The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T cells, PD-1 is expressed in activated B cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).

Database Links

UniProt ID: Q15116

Entrez-Gene Id: 5133

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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PD-1 (D3W4U) Rabbit mAb #15121