Render Target: STATIC
Render Timestamp: 2024-12-20T11:19:58.604Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:24:09.332
Product last modified at: 2024-05-30T07:03:37.813Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

PDE5 (P434) Antibody #3585

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 100
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PDE5 (P434) Antibody detects endogenous levels of total PDE5 protein. The antibody cross-reacts with a 35-40 kDa protein of unknown origin.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to central residues of human PDE5.

    Background

    The superfamily of phosphodiesterases (PDEs) catalyses the hydrolysis of 3',5'-cyclic nucleotides into the corresponding nucleotide 5'-monophosphates. PDE5 is a cGMP-specific enzyme that contains two cGMP binding sites in its amino-terminal regulatory domain (1). Elevation of cGMP levels causes activation of PKG, which phosphorylates PDE5 at Ser102 (2). Phosphorylation of PDE5 stimulates its enzymatic activity and enhances cGMP binding affinity in the regulatory domain, leading to a decrease in intracellular cGMP levels (3,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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