Render Target: STATIC
Render Timestamp: 2024-11-21T13:52:52.976Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:56:12.931
Product last modified at: 2024-10-10T20:30:08.992Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PDP1 (D8Y6L) Rabbit mAb #65575

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 55
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PDP1 (D8Y6L) Rabbit mAb recognizes endogenous levels of total PDP1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu327 of human PDP1 protein.

    Background

    Pyruvate dehydrogenase (PDH) catalyzes decarboxylation of the glycolytic intermediate pyruvate to acetyl-CoA (1). Acetyl-CoA is further metabolized in the tricarboxylic acid (TCA) cycle to generate ATP and NADH (1). Pyruvate dehydrogenase kinase 1 (PDHK1) phosphorylates PDH to suppress its activity, while pyruvate dehydrogenase phosphatase 1 (PDP1) dephosphorylates PDH to enhance its activity (1). Phosphorylation of PDP1 at Tyr94 inhibits PDP1 and has been shown to be present in a variety of cancer cell lines and primary human leukemia cells (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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