Render Target: STATIC
Render Timestamp:
3/26/2025, 10:14:40 AM EDT
3/26/2025, 2:14:40 PM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:15:44.833
Product last modified at: 2025-03-20T12:00:56.059Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. PGAM5 (Long Isoform) (E8C3L) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PGAM5 (Long Isoform) (E8C3L) Rabbit mAb #63454

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 28, 30
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PGAM5 (Long Isoform) (E8C3L) Rabbit mAb recognizes endogenous levels of total PGAM5 long isoform protein. This antibody specifically recognizes the PGAM5 long isoform (UniProt #Q96HS1-1) and does not recognize the PGAM5 short isoform (UniProt #Q96HS1-2).

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PGAM5 long isoform protein (UniProt #Q96HS1-1).

    Background

    Phosphoglycerate mutase family 5 (PGAM5) is a mitochondrial serine/threonine protein phosphatase (1,2). PGAM5 is required for necrosis induced by TNF-α or reactive oxygen species (2). In addition, PGAM5 dephosphorylates FUNDC1 to induce mitophagy under mitochondrial stresses (3). PGAM5 is also essential for RIPK1-mediated mitophagy activation during extracellular matrix (ECM) detachment of cancer cells (4). Furthermore, PGAM5 is cleaved by the protease presenilin-associated rhomboid-like protein (PARL) in response to mitochondrial stresses (such as induced by carbonyl cyanide m-chlorophenylhydrazone (CCCP) treatment). The cleaved PGAM5, released from the mitochondrial membrane, dephosphorylates β-catenin in the cytosol leading to its stabilization. Increased levels of β-catenin stimulate mitochondrial biogenesis (5).

    Database Links

    UniProt ID: Q96HS1


    Entrez-Gene Id: 192111


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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