Phospho-α-E-Catenin (Ser655/Thr658) Antibody #13231
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Research studies also suggest that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4).
Phosphorylation of α-E-catenin at Ser655 and Thr658 are post-translational modifications identified in a number of mass spectrometry studies, using a variety of tissue and cell types of both mouse and human origin (5-8).
- Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25.
- Yamada, S. et al. (2005) Cell 123, 889-901.
- Drees, F. et al. (2005) Cell 123, 903-15.
- Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65.
- Rigbolt, K.T. et al. (2011) Sci Signal 4, rs3.
- Brill, L.M. et al. (2009) Cell Stem Cell 5, 204-13.
- Huttlin, E.L. et al. (2010) Cell 143, 1174-89.
- Pan, C. et al. (2008) Proteomics 8, 4534-46.
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