Phospho-A20/TNFAIP3 (Ser381) Antibody #63523
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 92 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
A20 gets phosphorylated at Ser381 by IKKβ downstream of various innate immune activators, which enhances the deubiquitnase activiaty of A20 leading to disassembly of inflammatory signaling complexes (13). Therefore, A20 works as part of a negative feedback loop to limit NF-κB activation and inflammatory signaling (13). Phosphorylation at Ser381 could be used as a marker of active A20.
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- Tewari, M. et al. (1995) J Immunol 154, 1699-706.
- Jäättelä, M. et al. (1996) J Immunol 156, 1166-73.
- Laherty, C.D. et al. (1993) J Biol Chem 268, 5032-9.
- Hu, X. et al. (1998) Blood 92, 2759-65.
- Song, H.Y. et al. (1996) Proc Natl Acad Sci USA 93, 6721-5.
- Heyninck, K. and Beyaert, R. (1999) FEBS Lett 442, 147-50.
- Heyninck, K. et al. (1999) J Cell Biol 145, 1471-82.
- Evans, P.C. et al. (2004) Biochem J 378, 727-34.
- Lin, S.C. et al. (2008) J Mol Biol 376, 526-40.
- Hutti, J.E. et al. (2007) Mol Cell Biol 27, 7451-61.
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