Phospho-AML1 (Ser249) Antibody #4327
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Phospho-AML1 (Ser249) Antibody detects endogenous levels of AML1 protein only when phosphorylated on Ser249.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids around Ser249 of human AML1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).
- Wang, S. et al. (1993) Mol Cell Biol 13, 3324-39.
- Ogawa, E. et al. (1993) Proc Natl Acad Sci U S A 90, 6859-63.
- Okuda, T. et al. (1996) Cell 84, 321-30.
- Wang, Q. et al. (1996) Proc Natl Acad Sci U S A 93, 3444-9.
- North, T.E. et al. (2004) Stem Cells 22, 158-68.
- Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-87.
- Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79.
- Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25.
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