Phospho-ATF-2 (Thr69/71)/ATF-7 (Thr51/53) (E6A8A) Rabbit mAb #61584
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65, 75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:6400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:3200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #94125.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
ATF-7 is another member of the ATF/CREB family of leucine zipper proteins (5). Similarly, Thr51 and Thr53 (corresponding to Thr69 and Thr71 of ATF-2, respectively) can be phosphorylated under different conditions (6,7).
- Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.
- Gupta, S. et al. (1995) Science 267, 389-93.
- van Dam, H. et al. (1995) EMBO J 14, 1798-811.
- Livingstone, C. et al. (1995) EMBO J 14, 1785-97.
- Peters, C.S. et al. (2001) J Biol Chem 276, 13718-26.
- Camuzeaux, B. et al. (2008) J Mol Biol 384, 980-91.
- Maekawa, T. et al. (2010) EMBO J 29, 196-208.
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