Render Target: STATIC
Render Timestamp:
4/4/2025, 6:24:01 AM EDT
4/4/2025, 10:24:01 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:20:33.582
Product last modified at: 2025-03-20T14:45:11.833Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-BCL2L13 (Ser275) Antibody #39233

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 90
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-BCL2L13 (Ser275) Antibody recognizes endogenous levels of BCL2L13 protein only when phosphorylated at Ser275 of human BCL2L13 (equivalent to Ser272 of mouse BCL2L13).

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser275 of human BCL2L13 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    BCL2L13 (Bcl-2-like protein 13, Bcl-rambo) is an atypical member of the Bcl-2 family of proteins that regulate apoptosis at the mitochondria (1). While it contains conserved Bcl-2 homology (BH) domains -1, -2, -3, and -4, it has a unique C-terminal region and does not interact with other Bcl-2 family members (1). BCL2L13 may regulate apoptosis in a cellular context manner (reviewed in 2). It was originally identified as a pro-apoptotic protein with activity dependent on its C-terminal region (1). Interaction with the adenine nucleotide translocator (ANT) suppresses the ADP/ATP-dependent translocation activity of ANT (3). Other studies have found that BCL2L13 can inhibit apoptosis in glioblastoma and tumor growth (4). These studies found that BCL2L13 binds to pro-apoptotic ceramide synthases 2 (CerS2) and 6 (CerS6) and blocks homo- and heteromeric CerS2/6 complex formation and activity. Studies have also found that BCL2L13 can regulate mitochondrial fragmentation and can bind to LC3 to facilitate the clearance of damaged mitochondria through mitophagy (5,6).

    Phosphorylation of BCL2L13 at Ser275 plays a role in the clearance of damaged mitochondria through mitophagy and is important in maintaining cardiac function (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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