Phospho-c-Kit (Tyr568/570) Antibody #48347
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 145 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Tyr568 and Tyr570 are located in the juxtamembrane region of c-Kit. The phosphorylation of c-Kit at these sites is ligand induced and provides a docking site for recruitment of several adaptors/kinases, including Src, Cbl, SHP-1, APS, LNK, and SOSC6. Depending on which adaptor is bound, the outcome can lead to either cell survival and proliferation or c-Kit ubiquitination and degradation (8-13).
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- Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
- Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
- Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
- Nocka, K. et al. (1990) EMBO J 9, 1805-13.
- Hirota, S. et al. (1998) Science 279, 577-80.
- Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
- Phung, B. et al. (2011) PLoS One 6, e24064.
- Simon, C. et al. (2008) Blood 112, 4039-47.
- Masson, K. et al. (2006) Biochem J 399, 59-67.
- Bayle, J. et al. (2004) J Biol Chem 279, 12249-59.
- Wollberg, P. et al. (2003) Biochem J 370, 1033-8.
- Kozlowski, M. et al. (1998) Mol Cell Biol 18, 2089-99.
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