Phospho-c-Myc (Thr244) Antibody #62142
- WB
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Phosphorylation of c-Myc at Thr58 and Ser62 can control proteasomal-dependent degradation of the transcription factor. Phosphorylation of c-Myc at these sites is a stepwise process, whereby mitogens, mitosis, or cellular stress induce phosphorylation at Ser62, which serves as a priming site for GSK-3 phosphorylation of Thr58 (5-9). A second phosphodegron was identified at Thr244/Thr248 that acts cooperatively with Thr58/Ser62 to promote c-Myc ubiquitination and proteasomal degradation (10).
- Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
- Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
- Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
- Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.
- Lutterbach, B. and Hann, S.R. (1994) Mol Cell Biol 14, 5510-22.
- Gregory, M.A. et al. (2003) J Biol Chem 278, 51606-12.
- Yada, M. et al. (2004) EMBO J 23, 2116-25.
- Seo, H.R. et al. (2008) J Biol Chem 283, 15601-10.
- Benassi, B. et al. (2006) Mol Cell 21, 509-19.
- Welcker, M. et al. (2022) Sci Adv 8, eabl7872.
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