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Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:17:18.332
Product last modified at: 2025-04-01T19:30:10.064Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-CaMKK2 (Ser495) (E7W9S) Rabbit mAb #35392

Filter:
  • WB
Western Blotting Image 1: Phospho-CaMKK2 (Ser495) (E7W9S) Rabbit mAb
Western blot analysis of extracts from untreated LNCaP and SNB-19 cells, and mouse brain tissue, untreated (-) or treated with λ/CIP (+), using Phospho-CaMKK2 (Ser495) (E7W9S) Rabbit mAb (upper), CaMKK2 (D8D4D) Rabbit mAb #16810 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of CaMKK2 protein in SNB-19 cells is consistent with the predicted expression pattern.

To Purchase # 35392

Cat. # Size Qty. Price
35392T 20 µl
$173
35392S 100 µl
$401

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 68, 70
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-CaMKK2 (Ser495) (E7W9S) Rabbit mAb recognizes endogenous levels of CaMKK2 protein only when phosphorylated at Ser495. Bands of unknown origin are observed at 42 kDa and 25 kDa.

Species Reactivity:

Human, Mouse

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser495 of human CaMKK2 protein.

Background

Calcium/Calmodulin-dependent Protein Kinase Kinase 2 (CaMKK2) is a member of the CaMK family that contains a central Ser/Thr kinase domain followed by a regulatory domain consisting of overlapping autoinhibitory and CaM-binding regions (1). CaMKK2 can be distinguished from other CaMK family members by the presence of a unique Pro/Arg/Gly-rich insert following the ATP-binding domain (2). CaMKK2 phosphorylates CaMKI at Thr177 and CaMKIV at Thr200 (3). CaMKK2 also phosphorylates AMPKα in response to calcium (4). CaMKK2 has been implicated in long-term memory formation (5) and adipocyte development (6). CaMKK2 is phosphorylated at Ser511 by death-associated protein kinase (DAPK) in a signaling cascade thought to be involved in neuronal death (7).
Multiple CaMKK2 phosphorylation sites have been identified, including Ser495, and C-terminal phosphorylation of the CaMKK2 autoinhibitory region may regulate its function and localization (8).

Pathways

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