Phospho-CaMKK2 (Ser511) Antibody #12818
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 68 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser511 of human CaMKK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Calcium/Calmodulin-dependent Protein Kinase Kinase 2 (CaMKK2) is a member of the CaMK family that contains a central Ser/Thr kinase domain followed by a regulatory domain consisting of overlapping autoinhibitory and CaM-binding regions (1). CaMKK2 can be distinguished from other CaMK family members by the presence of a unique Pro/Arg/Gly-rich insert following the ATP-binding domain (2). CaMKK2 phosphorylates CaMKI at Thr177 and CaMKIV at Thr200 (3). CaMKK2 also phosphorylates AMPKα in response to calcium (4). CaMKK2 has been implicated in long-term memory formation (5) and adipocyte development (6). CaMKK2 is phosphorylated at Ser511 by death-associated protein kinase (DAPK) in a signaling cascade thought to be involved in neuronal death (7).
- Tokumitsu, H. et al. (1997) Biochemistry 36, 12823-7.
- Tokumitsu, H. et al. (1995) J Biol Chem 270, 19320-4.
- Anderson, K.A. et al. (1998) J Biol Chem 273, 31880-9.
- Hawley, S.A. et al. (2005) Cell Metab 2, 9-19.
- Peters, M. et al. (2003) J Neurosci 23, 9752-60.
- Lin, F. et al. (2011) Endocrinology 152, 3668-79.
- Schumacher, A.M. et al. (2004) Biochemistry 43, 8116-24.
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