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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-CARD11 (Ser652) Antibody #5189

Filter:
  • WB
Western Blotting Image 1: Phospho-CARD11 (Ser652) Antibody
Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #9905 (40 nM) and calcium ionophore A23187 (2 μM) for 15 minutes, and λ-phosphatase plus calf intestinal phosphatase (CIP) as indicated, using Phospho-CARD11 (Ser652) Antibody (upper) and CARD11 (1D12) Rabbit mAb #4435 (lower).

To Purchase # 5189

Cat. # Size Qty. Price
5189S 100 µl
$357

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 130
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-CARD11 (Ser652) Antibody detects endogenous levels of CARD11 protein only when phosphorylated at Ser652.

Species Reactivity:

Human, Mouse, Rat

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to a region surrounding Ser652 of human CARD11 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

CARD11/Carma1/Bimp3 belongs to the MAGUK (membrane-associated guanylate kinase) family that typically function as molecular scaffolds in the assembly of multiprotein complexes (1,2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-κB activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-κB (1,2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3,4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-κB activation and lymphocyte signaling (4-7).
Phosphorylation at multiple sites within the central region of CARD11 regulates NF-κB activation (8-10).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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