Render Target: STATIC
Render Timestamp: 2024-12-12T11:13:04.799Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-08-01 15:25:54.868
Product last modified at: 2024-12-04T14:30:08.591Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. Phospho-cdc2 (Tyr15) Antibody

Phospho-cdc2 (Tyr15) Antibody #9111

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk Dm X Sc
    SENSITIVITY Endogenous
    MW (kDa) 34
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Dm-D. melanogaster 
    • X-Xenopus 
    • Sc-S. cerevisiae 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-cdc2 (Tyr15) Antibody detects endogenous levels of cdc2, CDK2 and CDK5 only when phosphorylated at tyrosine 15. Based on sequence similarity, the antibody may also cross-react with CDK3. The antibody does not cross-react with CDK4, CDK6 or CDK7. It does detect the yeast orthologue of cdc2 (cdc28) when phosphorylated at tyrosine 19.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, D. melanogaster, Xenopus, S. cerevisiae

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr15 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).

    Database Links

    UniProt ID: P06493


    Entrez-Gene Id: 983


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