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XML generation date: 2025-03-07 13:12:21.289
Product last modified at: 2025-04-01T15:15:10.038Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-CRMP-2 (Thr514) Antibody #9397

Filter:
  • WB
Western Blotting Image 1: Phospho-CRMP-2 (Thr514) Antibody
Western blot analysis of extracts from PC12 cells, untreated or λ phosphatase treated, using Phospho-CRMP-2 (Thr514) Antibody (upper) or CRMP-2 Antibody #9393 (lower).

To Purchase # 9397

Cat. # Size Qty. Price
9397S 100 µl
$357

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 60-80
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-CRMP-2 (Thr514) Antibody detects endogenous levels of CRMP-2 only when phosphorylated at Thr514.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr514 of human CRMP-2. Antibodies are purified by peptide affinity chromatography.

Background

Collapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5).

Pathways

Explore pathways related to this product.


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