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Phospho-dCK (Ser74) Antibody

Phospho-dCK (Ser74) Antibody #40371

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	30
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-dCK (Ser74) Antibody recognizes endogenous levels of dCK protein only when phosphorylated at Ser74.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser74 of human dCK protein. Antibodies are purified by peptide affinity chromatography.

Background

Deoxycytidine kinase (dCK) catalyzes the rate-limiting step in the nucleoside salvage pathway. dCK phosphorylates salvaged deoxycytidine (dC) to generate deoxycytidine monophosphate (dCMP), a precursor for both deoxycytidine triphosphate (dCTP) and deoxythymidine triphosphate (dTTP) pools (1). dCK also phosphorylates deoxyadenosine (dA) and deoxyguanosine (dG) to generate dA monophosphate and dG monophosphate, respectively. Gemcitabine is an anticancer nucleoside that is a dC analog. It is a pro-drug and must be phosphorylated by dCK to an active form. dCK is phosphorylated on Ser74 by the DNA damage signaling kinases ATM and ATR, and this phosphorylation increases dCK activity (2,3).

Database Links

UniProt ID: P27707

Entrez-Gene Id: 1633

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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