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Phospho-delta-Opioid Receptor (Ser363) Antibody

Phospho-delta-Opioid Receptor (Ser363) Antibody #3641

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Inquiry Info. # 3641

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Supporting Data

REACTIVITY	H
SENSITIVITY	Transfected Only
MW (kDa)	50 to 65
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-delta-Opioid Receptor (Ser363) Antibody detects transfected delta-opioid receptor only when phosphorylated at serine 363.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around serine 363 of human delta-opioid receptor. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Phosphorylation is an essential signal involved in desensitization and internalization of G-protein-coupled receptors (GPCRs) (1). The opioid receptors, like other GPCRs, are typically phosphorylated at multiple sites upon agonist binding (2,3). However, little is known about the specific role of each phosphorylation site in GPCR desensitization and internalization or about how the kinetics of phosphate addition affects these important processes. Phosphorylation of the delta-opioid receptor (DOR) is hierarchical and involves at least two sites: threonine 358 and serine 363 (4). Serine 363 appears to be the primary phosphorylation site induced by the DOR-specific agonist DPDPE (4). Phosphorylation of DOR at serine 363 correlates with receptor internalization (4). FMRP was suggested to associate with the RNA interference machinery (4).

Database Links

UniProt ID: P41143

Entrez-Gene Id: 4985

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For Research Use Only. Not For Use In Diagnostic Procedures.

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