Render Target: STATIC
Render Timestamp: 2025-01-03T10:50:05.348Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-09-19 13:01:10.820
Product last modified at: 2025-01-01T09:02:38.550Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-Desmoplakin (Ser165/166) Antibody #5885

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 320, 250
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Desmoplakin (Ser165/166) Antibody recognizes endogenous levels of desmoplakin protein only when phosphorylated at Ser165/166.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser165/166 of human desmoplakin protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Desmosomes are a class of intracellular junctions that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium (1). They derive their characteristic strength from the protein desmoplakin, which acts as a tether by binding the cytoplasmic component of the desmosome at its N-terminus (2) while its C-terminus is anchored to the intermediate-filament cytoskeleton (3). This association mitigates the impact of mechanical forces on the desmosome by distributing them throughout the cytoskeleton and tissue (4). Desmoplakin is essential for normal desmosomal adhesion (5); defects can result in pathologies that include cardiomyopathy (6), keratoderma (7), or the skin blistering disease Epidermolysis bullosa (8).

    Phospho-Desmoplakin (Ser165/166) Antibody is directed against a site that was identified at Cell Signaling Technology (CST) using PTMScan®, our LC-MS/MS platform for modification site discovery. Please visit PhosphoSitePlus®, a modification site knowledgebase developed and maintained by CST, at www.phosphosite.org for more information.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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