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PDP - Template Name: IHC Kit
PDP - Template ID: *******35f95d7
  1. Products /
  2. Primary Antibodies /
  3. SignalStain® Phospho-ErbB Family IHC Sampler Kit

SignalStain® Phospho-ErbB Family IHC Sampler Kit #8111

    Product Information

    Product Description

    The SignalStain® Phospho-ErbB Family IHC Sampler Kit from Cell Signaling Technology allows the researcher to examine paraffin-embedded tissues or cells with antibodies that will detect active ErbB 1, 2 and 3 as well as total epidermal growth factor receptor (EGFR). Each antibody is validated for use in immunohistochemical assays using multiple approaches. Also included in the kit are control slides that can be used to verify the performance of each antibody and a primary antibody diluent. See the table above for the recommended antibody diluent for each antibody provided in the kit.

    Specificity / Sensitivity

    Each antibody in the SignalStain® Phospho-ErbB Family IHC Sampler Kit detects endogenous levels of its target protein. Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb detects endogenous EGF receptor only when phosphorylated at Tyr1068. This antibody may cross-react weakly with other tyrosine-phosphorylated proteins. Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb detects endogenous levels of ErbB2 only when phosphorylated at Tyr1221/1222. The antibody does not cross react with other activated ErbB family members or other tyrosine-phosphorylated proteins. Phopho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb detects endogenous levels of HER3/ErbB3 proteins only when phosphorylated at tyrosine 1289. The antibody does not cross-react with other phosphorylated receptor tyrosine kinases at significant level. EGF Receptor (D38B1) Rabbit mAb detects endogenous levels of total EGF receptor protein.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr1068 of human EGFR, Tyr1221/1222 of human ErbB2, or to Tyr1289 of human HER3/ErbB3. Monoclonal antibody to EGFR is produced by immunizing animals with a synthetic GST-fusion protein corresponding to residues containing the cytoplasmic domain of human EGF receptor.

    Background

    The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling and lysosomal degradation (1,2). EGFR is phosphorylated on multiple tyrosine residues, each of which leads to activation of a specific downstream pathway. Major residues involved in EGFR signaling include: Tyr845, Tyr992, Tyr1045, Tyr1068, Tyr1148 and Tyr1173 (2-9). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; a mutation to either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).
    The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (11). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (12). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers, as such it is a key therapeutic target (13). ErbB2 has several key residues that are phosphorylated upon its activation including Tyr877, Tyr1221/1222 and Tyr1248 (11,14).
    HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Ligand binding promotes formation of a heterodimer containing ErbB3 and another ErbB protein and subsequent tyrosine phosphorylation of ErbB3 by the activated ErbB kinase (15,16). At least nine putative carboxy-terminal tail tyrosine phosphorylation sites are found in ErbB3, including Tyr1222 and Tyr1289 (17). ErbB3 may function as an oncogenic unit together with other ErbB members in tumor development; ErbB2 requires ErbB3 to drive breast tumor cell proliferation (18). A novel anti-tumor strategy involves inhibiting the interaction between ErbB3 and ErbB tyrosine kinases.
    1. Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9.
    2. Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12.
    3. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4.
    4. Hubbard, S.R. et al. Nature 372, 746-54.
    5. Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43.
    6. Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.
    7. Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40.
    8. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66.
    9. Rojas, M. et al. (1996) J Biol Chem 271, 27456-61.
    10. Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.
    11. Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57.
    12. Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4.
    13. Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.
    14. Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9.
    15. Yarden, Y. and Sliwkowski, M.X. (2001) Nat Rev Mol Cell Biol 2, 127-37.
    16. Guy, P.M. et al. (1994) Proc Natl Acad Sci USA 91, 8132-6.
    17. Kim, H.H. et al. (1994) J Biol Chem 269, 24747-55.
    18. Holbro, T. et al. (2003) Proc Natl Acad Sci USA 100, 8933-8.

    Database Links

    UniProt ID: P21860 , P00533 , P04626


    Entrez-Gene Id: 2065 , 1956 , 2064


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    SignalStain is a registered trademark of Cell Signaling Technology, Inc.
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