Phospho-Ezh2 (Thr345) (E7T7K) Rabbit mAb #45153
- WB
- IP
- ChIP
- C&R
Supporting Data
REACTIVITY | H M R Mk |
SENSITIVITY | Endogenous |
MW (kDa) | 98 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Chromatin IP | 1:50 |
Chromatin IP-seq | 1:50 |
CUT&RUN | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Ezh2 is phosphorylated on Thr345 by cyclin-dependent kinase 1 (CDK1), which increases targeting of Ezh2 to its target loci and promotes methylation of H3 at Lys27 (7,8). This activated form of Ezh2 may deposit initial H3K27me3 marks onto newly synthesized daughter strands of DNA during replication (9). In addition, phosphorylation of Thr345 affects Ezh2 stability by promoting its ubiquitination and subsequent degradation (9).
- Sellers, W.R. and Loda, M. (2002) Cancer Cell 2, 349-50.
- Visser, H.P. et al. (2001) Br J Haematol 112, 950-8.
- Chen, H. et al. (1996) Genomics 38, 30-7.
- Tonini, T. et al. (2004) Oncogene 23, 4930-7.
- Müller, J. et al. (2002) Cell 111, 197-208.
- Kleer, C.G. et al. (2003) Proc Natl Acad Sci U S A 100, 11606-11.
- Chen, S. et al. (2010) Nat Cell Biol 12, 1108-14.
- Kaneko, S. et al. (2010) Genes Dev 24, 2615-20.
- Wu, S.C. and Zhang, Y. (2011) J Biol Chem 286, 28511-9.
Limited Uses
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