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Render Timestamp:
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4/5/2025, 3:17:51 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:08:22.362
Product last modified at: 2024-09-13T07:01:31.565Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-FADD (Ser191) Antibody (Mouse Specific) #2785

Filter:
  • WB
Western Blotting Image 1: Phospho-FADD (Ser191) Antibody (Mouse Specific)
Western blot analysis of extracts from A20 and L929 cells treated with 4 mM hydroxyurea or 1 µg/ml nocodazole (each for 20 hours), using Phospho-FADD (Ser191) Antibody (Mouse Specific).
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Inquiry Info. # 2785

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Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 30
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • M-Mouse 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-FADD (Ser191) Antibody (Mouse Specific) detects endogenous levels of mouse FADD protein only when phosphorylated at serine 191.

Species Reactivity:

Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser191 of mouse FADD. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase-8 (1,2). FADD has a carboxy-terminal death domain, which interacts with the cytoplasmic tail of the membrane receptor, and an amino-terminal death effector domain, which interacts with caspase-8. Clustering of the receptors upon stimulation brings about FADD and caspase-8 oligomerization, activating the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194, while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle-dependent (3) and may be related to its regulatory role in embryonic development and cell cycle progression (4,5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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