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Product last modified at: 2025-01-01T09:03:20.883Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-FLT3 (Tyr589/591) (E4D6Y) Rabbit mAb #60413

Filter:
  • WB
  • IP
  • IF
  • F
Western Blotting Image 1: Phospho-FLT3 (Tyr589/591) (E4D6Y) Rabbit mAb
Western blot analysis of extracts from SEM cells, vehicle-treated (-) or treated with PKC412 #9493 (1 μM, 24 hr; +), using Phospho-FLT3 (Tyr589/591) (E4D6Y) Rabbit mAb (upper) and FLT3 (8F2) Rabbit mAb #3462 (lower). The loss of signal corresponding to phospho-FLT3 (Tyr589/591) after treating the cells with FLT3 kinase inhibitor PKC412 demonstrates specificity of the antibody for phosphorylated FLT3.

To Purchase # 60413

Cat. # Size Qty. Price Ships
60413T 20 µl
$173
60413S 100 µl
$401

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 160
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (Immunocytochemistry) 1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #48437.

Protocol

Specificity / Sensitivity

Phospho-FLT3 (Tyr589/591) (E4D6Y) Rabbit mAb recognizes endogenous levels of FLT3 protein only when doubly phosphorylated at Tyr589 and Tyr591.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic double-phospho-peptide corresponding to residues surrounding Tyr589 and Tyr591 of human FLT3 protein.

Background

FMS-related tyrosine kinase 3 (FLT3, also called FLK2) is a member of the Type III receptor tyrosine kinase family, which includes c-Kit, PDGFR, and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream substrates (3). The p85 subunit of PI3 kinase, SHP2, GRB2, and Shc have all been reported to associate with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).

Pathways

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For Research Use Only. Not For Use In Diagnostic Procedures.
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