Phospho-Glucocorticoid Receptor (Ser226) (D9D3V) Rabbit mAb #97285
- WB
- IP
- IF
- ChIP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 94, 91 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
| Chromatin IP | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Phosphorylation of GR at serine 226 by JNK enhances nuclear export after ligand depletion (6,7). Phosphorylation of various serine residues, including serine 226 also affect GR binding to different target genes, contributing to an additional layer of transcriptional regulation (8). Serine 226 phosphorylation has also been linked to depression disorders as well as inflammation (9-11).
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- Necela, B.M. and Cidlowski, J.A. (2003) Trends Pharmacol. Sci. 24, 58-61.
- Wang, Z. et al. (2002) J. Biol. Chem. 277, 26573-80.
- Rogatsky, I. et al. (1998) J. Biol. Chem. 273, 14315-21.
- Krstic, M. D. et al. (1997) Mol. Cell. Biol. 17, 3947-54.
- Rogatsky, I. et al. (1998) Proc Natl Acad Sci U S A 95, 2050-5.
- Itoh, M. et al. (2002) Mol Endocrinol 16, 2382-92.
- Blind, R.D. and Garabedian, M.J. (2008) J Steroid Biochem Mol Biol 109, 150-7.
- Mitic, M. et al. (2013) Neuropharmacology 70, 100-11.
- Lukic, I. et al. (2015) J Mol Neurosci 55, 951-8.
- Verhoog, N.J. et al. (2011) J Biol Chem 286, 19297-310.
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