Phospho-HER2/ErbB2 (Thr686) Antibody #88583
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 185 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Thr686 is located in the cytoplasmic juxtamembrane region of ErbB2. PKC, PKA, and PKG II kinases have been reported to phosphorylate ErbB2 at this site, but the functional consequences of phosphorylation may be context dependent. For example, one research study reported that phosphorylation of ErbB2 at Thr686 enhanced ligand-induced receptor activation (6), while a separate research study reported inhibitory effects of phosphorylation on ErbB2 activation (8).
- Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57.
- Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4.
- Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.
- Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8.
- Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9.
- Monje, P.V. et al. (2008) J Biol Chem 283, 34087-100.
- Ouyang, X. et al. (1998) Carcinogenesis 19, 2013-9.
- Zhu, M. et al. (2016) Mol Med Rep 13, 1909-13.
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