Phospho-HER2/ErbB2 (Thr701) (E8Q6O) Rabbit mAb #56277
- WB
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 185 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Thr701 is located in the cytoplasmic juxtamembrane region of HER2/ErbB2. HER2/ErbB2 Thr701 was demonstrated as a direct phosphorylation target of ERK1/2. The phosphorylation of Thr701 is a common mechanism for the negative feedback control of active ErbB receptor dimers, such as ErbB2/EGFR or ErbB2/ErbB3 (6-8).
- Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57.
- Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4.
- Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4.
- Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8.
- Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9.
- Kawasaki, Y. et al. (2016) Sci Rep 6, 31502.
- Chen, C.H. et al. (2017) Mol Oncol 11, 1273-1287.
- Park, C.M. et al. (2018) Oncol Lett 15, 1758-1762.
Limited Uses
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