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Render Timestamp: 2024-11-21T13:56:29.580Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:56:52.728
Product last modified at: 2024-11-18T22:45:08.242Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Phospho-IKKε (Ser172) (D1B7) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-IKKε (Ser172) (D1B7) Rabbit mAb #8766

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-IKKε (Ser172) (D1B7) Rabbit mAb recognizes endogenous levels of IKKε protein only when phosphorylated at Ser172. This antibody may cross-react with phospho-TBK1/NAK.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse, Rat, Monkey, Dog

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human IKKε protein.

    Background

    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
    Recently, two homologs of IKKα and IKKβ have been described, called IKKε (also known as IKK-i) and TBK1 (also known as T2K or NAK), and activation of either of these kinases results in NF-κB activation. IKKε contains the kinase domain in its amino terminus, which shares 30% identity to that of IKKα or IKKβ. IKKε is expressed mainly in immune cells, and may play a special role in the immune response (14-18). IKKε and TBK1 kinase capabilities are activated by phosphorylation at Ser172 within their activation loops (19). IRF-3, a substrate for IKKε and TBK1, plays a critical role in innate immune responses (20).
    1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
    2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
    3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
    4. Brown, K. et al. (1995) Science 267, 1485-8.
    5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
    6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
    7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
    8. Zandi, E. et al. (1997) Cell 91, 243-52.
    9. Karin, M. (1999) Oncogene 18, 6867-74.
    10. DiDonato, J.A. et al. (1997) Nature 388, 548-54.
    11. Mercurio, F. et al. (1997) Science 278, 860-6.
    12. Johnson, L.N. et al. (1996) Cell 85, 149-58.
    13. Delhase, M. et al. (1999) Science 284, 309-13.
    14. Shimada, T. et al. (1999) Int Immunol 11, 1357-62.
    15. Peters, R.T. et al. (2000) Mol Cell 5, 513-22.
    16. Tojima, Y. et al. (2000) Nature 404, 778-82.
    17. Bonnard, M. et al. (2000) EMBO J 19, 4976-85.
    18. Peters, R.T. and Maniatis, T. (2001) Biochim Biophys Acta 1471, M57-62.
    19. Kishore, N. et al. (2002) J Biol Chem 277, 13840-7.
    20. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.

    Database Links

    UniProt ID: Q14164


    Entrez-Gene Id: 9641


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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