Phospho-IRF-3 (Ser396) Antibody #4961
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Product Name
- WB
Inquiry Info. # 4961
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Supporting Data
| REACTIVITY | H |
| SENSITIVITY | |
| MW (kDa) | 57 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
IRF3 can inhibit cell growth and plays a critical role in controling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF3, leading to its translocation to the nucleus, where it activates promoters containing IRF3-binding sites. Phosphorylation of IRF3 occurs at a cluster of serine and threonine residues in the carboxy-terminus between residues 385 and 405 leading to its association with the coactivator protein, p300/CBP which promotes DNA binding and transcriptional activity (5). During infection, IRF3 is likely activated through a pathway that includes activation of Toll-like receptors and of a kinase complex that includes IKKe and TBK1 (6,7). IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double stranded RNA treatment, and these events play a role in its activation (8).
- Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
- Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
- Hiscott, J. et al. (1999) J Interferon Cytokine Res 19, 1-13.
- Kim, T.Y. et al. (2003) J Biol Chem 278, 15272-8.
- Yoneyama, M. et al. (2002) J Interferon Cytokine Res 22, 73-6.
- Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
- Kopp, E. and Medzhitov, R. (2003) Curr Opin Immunol 15, 396-401.
- Servant, M.J. et al. (2003) J Biol Chem 278, 9441-7.
Limited Uses
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