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Render Timestamp: 2024-11-20T11:25:12.454Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:55:54.767
Product last modified at: 2024-10-17T16:30:09.907Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb #5610

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 180
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb recognizes endogenous levels of IRS-1 protein only when phosphorylated at Ser318. Note: Ser318 is the mouse residue; the corresponding human residue is Ser323. The antibody cross reacts with a inducible nonspecific band at around 57 kDa.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser318 of mouse IRS-1 protein.

    Background

    Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).
    PKC phosphorylates mouse IRS-1 at Ser318 (human Ser323) by insulin receptor activation or by other stimulation such as TPA, IL-6, retinoic acid treatment (11-14) . The phosphorylation at Ser318 acts as a negative feedback signal to down regulates of insulin effect (14-16).
    1. Sun, X.J. et al. (1991) Nature 352, 73-77.
    2. Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672.
    3. Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.
    4. Wang, L.M. et al. (1993) Science 261, 1591-1594.
    5. Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189.
    6. Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121.
    7. Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
    8. Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645.
    9. De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947.
    10. Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.
    11. Greene, M.W. et al. (2004) Biochem J 378, 105-116.
    12. Weigert, C. et al. (2006) J Biol Chem 281, 7060-7067.
    13. del Rincón, S.V. et al. (2004) Oncogene 23, 9269-9279.
    14. Moeschel, K. et al. (2004) J Biol Chem 279, 25157-25163.
    15. Weigert, C. et al. (2005) J Biol Chem 280, 37393-37399.
    16. Hennige, A.M. et al. (2006) FASEB J 20, 1206-1208.

    Database Links

    UniProt ID: P35568


    Entrez-Gene Id: 3667


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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