Phospho-Jak Family Antibody Sampler Kit #97999
Product Information
Kit Usage Information
Protocols
- 3230: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin)
- 3344: Western Blotting, Immunohistochemistry (Paraffin)
- 5031: Western Blotting
- 7074: Western Blotting
- 8082: Western Blotting
- 8827: Western Blotting, Immunoprecipitation (Agarose)
- 14193: Western Blotting, Immunoprecipitation (Agarose)
- 68790: Western Blotting
- 74129: Western Blotting
Product Description
Specificity / Sensitivity
Each antibody in the Phospho-Jak Family Antibody Sampler kit detects endogenous levels of its target protein. Phospho-Jak1 (Tyr1034/1035) (D7N4Z) Rabbit mAb can detect Jak1 when dually or singly phosphorylated at Tyr1034. This site has historically been referenced as Tyr1022 and Ty1023. Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb can detect Jak2 when phosphorylated at Tyr1008 or when dually phosphorylated at Tyr1007 and Tyr1008. Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb can also detect Jak3 when dually phosphorylated or when phosphorylated at each of the single phosphorylation sites. Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb detects Tyk2 when phosphorylated at both Tyr1054 and Tyr1055. Cross-reactivity was not observed with other family members.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Ile800 of Jak1, Pro841 of Jak2, and the carboxy terminus of Jak3 and Tyk2. Phosphorylation-specific monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Tyr1034/1035 of Jak1, Tyr1008 of Jak2, Tyr980/981 of Jak3, and Tyr1054/1055 of Tyk2.
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phosphotyrosine-binding domains.
Activation of Jak kinases upon cytokine receptor binding is associated with tyrosine phosphorylation within their activation loops, including Tyr1034/1035 of Jak1, Tyr1007/1008 of Jak2, Tyr980/981 of Jak3, and Tyr1054/1055 of Tyk2. Many studies have indicated that various cytokine receptors have clear preferences that utilize distinct Jak family members. Aberrant regulation of Jak signaling is associated with a number of diseases, including myeloproliferative neoplasms, leukemia, and inflammatory disease (6).
- Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-5.
- VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
- Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
- Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
- Babon, J.J. et al. (2014) Biochem J 462, 1-13.
Limited Uses
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