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Render Timestamp: 2024-12-20T11:16:58.867Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-18 16:01:07.552
Product last modified at: 2024-12-17T12:45:16.323Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Phospho-Jak Family Antibody Sampler Kit

Phospho-Jak Family Antibody Sampler Kit #97999

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Phospho-Jak Family Antibody Sampler Kit provides an economical means of detecting the activation of Jak family members using phospho-specific and control antibodies. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the Phospho-Jak Family Antibody Sampler kit detects endogenous levels of its target protein. Phospho-Jak1 (Tyr1034/1035) (D7N4Z) Rabbit mAb can detect Jak1 when dually or singly phosphorylated at Tyr1034. This site has historically been referenced as Tyr1022 and Ty1023. Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb can detect Jak2 when phosphorylated at Tyr1008 or when dually phosphorylated at Tyr1007 and Tyr1008. Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb can also detect Jak3 when dually phosphorylated or when phosphorylated at each of the single phosphorylation sites. Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb detects Tyk2 when phosphorylated at both Tyr1054 and Tyr1055. Cross-reactivity was not observed with other family members.

    Source / Purification

    Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Ile800 of Jak1, Pro841 of Jak2, and the carboxy terminus of Jak3 and Tyk2. Phosphorylation-specific monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Tyr1034/1035 of Jak1, Tyr1008 of Jak2, Tyr980/981 of Jak3, and Tyr1054/1055 of Tyk2.

    Background

    Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phosphotyrosine-binding domains.
    Activation of Jak kinases upon cytokine receptor binding is associated with tyrosine phosphorylation within their activation loops, including Tyr1034/1035 of Jak1, Tyr1007/1008 of Jak2, Tyr980/981 of Jak3, and Tyr1054/1055 of Tyk2. Many studies have indicated that various cytokine receptors have clear preferences that utilize distinct Jak family members. Aberrant regulation of Jak signaling is associated with a number of diseases, including myeloproliferative neoplasms, leukemia, and inflammatory disease (6).

    Database Links

    UniProt ID: P23458 , P29597 , P52333 , O60674


    Entrez-Gene Id: 3716 , 7297 , 3718 , 3717


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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