Render Target: STATIC
Render Timestamp: 2024-11-21T13:41:24.611Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:56:33.760
Product last modified at: 2024-11-18T13:15:12.027Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb #8082

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 125
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb recognizes endogenous levels of Jak2 protein only when phosphorylated at Tyr1008. This antibody also reacts with Jak2 when dually phosphorylated at Tyr1007 and Tyr1008. Cross-reactivity was not observed with other Jak family members by western blot.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Rat, Monkey, Xenopus, Bovine, Pig

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr1008 of human Jak2 protein.

    Background

    Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
    Jak2 signaling is associated with a number of cytokines, growth factors, and hormones including IL-3, IL-5, IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), erythropoietin (EPO), thrombopoietin (TPO) growth hormone, prolactin, and leptin (6-13). The oncogenic potential of Jak2 has been realized though translocations and point mutations resulting in its enhanced, de-regualated kinase activity, making Jak2 a potential therapeutic target. Jak2 gene translocations resulting in fusions with the TEL (TEL-JAK2) and PCM1 (PCM1-JAK2) have been found in leukemia patients (14,15). An activating point mutation in Jak2 resulting in a valine to phenylalanine switch at position 617 (V617F) has been implicated in myeloproliferative disorders including polycythemia vera and essential thrombocythemia (16).
    1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
    2. Darnell, J.E. (1997) Science 277, 1630-5.
    3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
    4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
    5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
    6. Quelle, F.W. et al. (1994) Mol Cell Biol 14, 4335-41.
    7. Witthuhn, B.A. et al. (1993) Cell 74, 227-36.
    8. Tortolani, P.J. et al. (1995) Blood 85, 3444-51.
    9. Pallard, C. et al. (1995) EMBO J 14, 2847-56.
    10. Narazaki, M. et al. (1994) Proc Natl Acad Sci USA 91, 2285-9.
    11. Argetsinger, L.S. et al. (1993) Cell 74, 237-44.
    12. Rui, H. et al. (1994) J Biol Chem 269, 5364-8.
    13. Ghilardi, N. and Skoda, R.C. (1997) Mol Endocrinol 11, 393-9.
    14. Lacronique, V. et al. (1997) Science 278, 1309-12.
    15. Reiter, A. et al. (2005) Cancer Res 65, 2662-7.
    16. Kralovics, R. et al. (2005) N Engl J Med 352, 1779-90.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Jak antibodies produced under license (granting certain rights including those under U.S. Patent No. 5,658,791) from Chemicon International, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.