Name: Phospho-LAT (Tyr171) Antibody
Price: 353.00 USD
Availability: InStock

Filter:

WB

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	36, 38
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-LAT (Tyr171) Antibody detects endogenous levels of LAT only when phosphorylated at tyrosine 171. Tyr171 of LAT, short isoform corresponds to Tyr200 of LAT, long isoform.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr171 of human LAT. Antibodies are purified by protein A and peptide affinity chromatography.

Background

LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4).

Database Links

UniProt ID: O43561

Entrez-Gene Id: 27040

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Phospho-LAT (Tyr171) Antibody #3581