Phospho-LAT (Tyr255) Antibody #45170
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Research studies have shown that LAT is also phosphorylated at Tyr225 by ZAP-70 and Syk following activation of the TCR, which facilitates its binding to Grb2 and Vav and subsequent downstream activation of ERK (5-7).
- Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-83.
- Zhang, W. et al. (1998) Cell 92, 83-92.
- Paz, P. E. et al. (2001) Biochem. J. 356, 461-71.
- Zhang, W. et al. (2000) J. Biol. Chem. 275, 23355-61.
- Zhang, W. et al. (2000) J Biol Chem 275, 23355-61.
- Paz, P.E. et al. (2001) Biochem J 356, 461-71.
- Lin, J. and Weiss, A. (2001) J Biol Chem 276, 29588-95.
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