Phospho-MEK1 (Ser298) (D1P9E) Rabbit mAb #98195
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
MEK1 is phosphorylated at Ser298 by PAK1, which facilitates signal transduction from Raf to MEK1 and Erk2 (5-7). MEK1 is also phosphorylated by cdk5 at Thr286 in mitotic cells, causing negative feedback of the p44/42 MAP kinase pathway (8).
- Crews, C.M. et al. (1992) Science 258, 478-480.
- Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19.
- Rosen, L.B. et al. (1994) Neuron 12, 1207-21.
- Cowley, S. et al. (1994) Cell 77, 841-52.
- Xu Be et al. (2001) J Biol Chem 276, 26509-15.
- Coles, L.C. and Shaw, P.E. (2002) Oncogene 21, 2236-44.
- Eblen, S.T. et al. (2002) Mol Cell Biol 22, 6023-33.
- Sharma, P. et al. (2002) J Biol Chem 277, 528-34.
Limited Uses
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