Phospho-MEK1 (Thr292) (D5L3K) Rabbit mAb #26975
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
In response to integrin signaling, p21-activated kinase-1 (PAK-1) phosphorylates MEK1 at Ser298, which enhances MEK1-ERK2 complex formation and MEK1 activation by Raf-1. These events positively regulate the Raf-1-MEK-ERK signaling cascade (5-9). Research studies have identified a negative regulatory loop in the Raf-1-MEK-ERK signaling cascade, in which ERK2-dependent phosphorylation of MEK1 at Thr292 negatively regulates MEK1 activation following cell adhesion. Specifically, ERK2-dependent phosphorylation of MEK1 also attenuates its PAK-1-mediated phosphorylation, contributing to a reduction in Raf-dependent phosphorylation of residues within the MEK1 activation loop (7,10).
- Crews, C.M. et al. (1992) Science 258, 478-480.
- Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19.
- Rosen, L.B. et al. (1994) Neuron 12, 1207-21.
- Cowley, S. et al. (1994) Cell 77, 841-52.
- Frost, J.A. et al. (1997) EMBO J 16, 6426-38.
- Rossomando, A.J. et al. (1994) Mol Cell Biol 14, 1594-602.
- Eblen, S.T. et al. (2004) Mol Cell Biol 24, 2308-17.
- Eblen, S.T. et al. (2002) Mol Cell Biol 22, 6023-33.
- Coles, L.C. and Shaw, P.E. (2002) Oncogene 21, 2236-44.
- Xu, B.e. et al. (1999) J Biol Chem 274, 34029-35.
Limited Uses
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